IN VITRO PROPAGATION OF INDIGENOUS PHU THO’S 5-PETALED WHITE ORCHID (Dendrobium anosmum Lindl.)
Từ khóa:
Coir, in vitro propagation, multi-shooting, orchid, Phu Tho’s 5-petaled white orchidTóm tắt
Indigenous Phu Tho’s 5-petaled white orchid (Dendrobium anosmum Lindl.) was propagated by an in vitro method from keikis. Keikis were sterilized with HgCl2 0.1% for 12 minutes, the regeneration rate reached 24.44%. The percentage of budding from keiki parts reached 100% on ½ MS medium with 1.0 mg/L BAP. The best multi-shooting obtained on ½ MS medium with 1.5 mg/L BAP and 0.5 mg/L TDZ, shoot multiplication corresponded to 3.57 times. The ½ MS medium with 0.5 mg/L TDZ and 1.0 mg/L 2,4D was optimal for callus formation from stems (45.18%), shoot tips (85.93%) and leaves (17.04%). The ½ MS medium containing 1.0 mg/L BAP and 0.2 mg/L NAA induced the highest rate of rooting (95.56%). The best acclimatization substrate including 25% of coir, 75% of fir bark was found the most suitable medium for plantlets with a 77.04% survival rate, 4.24 cm in plantlet height, 6.3 number of leaves per plantlet among 8 weeks in the net house.
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